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Cultural conditions for the biopolymer production by an alkali tolerant Bacillus -sp: isolated from soil were investigated and determination of optimal conditions was carried out by rsponse surface method. The maximal production of biopolymer was obtained after cultivation at 30 for 36 hrs in the mixture of 8% soluble starch, 0.75% yeast extract, 0.1% NaN0₃, 0.05% MgSO₄ 7H₂O and 1% Na₂CO₃ adjusted to pH 10. Under these conditions, about 44 g/l of biopolymer wece´produced. From the results of response surface analysis, optimal condition for the production of biopolymer were obtained at stationary point with 15.16 of C/N ratio, 34.62℃ of temperature and 9.50 of pH. On the basis of these conditions, it was estimated that 66.84 g/l of the biopolymer could be produced.
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